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Free Radicals and Antioxidant Protocols pp 403–417Cite as

Oxidized Low-Density Lipoprotein

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 610))

Abstract

Oxidized low-density lipoprotein (Ox-LDL) has been studied for over 25 years. Numerous pro- and anti-atherogenic properties have been attributed to Ox-LDL. Yet, Ox-LDL has neither been defined nor characterized, as its components and composition change depending on its source, method of preparation, storage, and use. It contains unoxidized and oxidized fatty acid derivatives both in the ester and free forms, their decomposition products, cholesterol and its oxidized products, proteins with oxidized amino acids and cross-links, and polypeptides with varying extents of covalent modification with lipid oxidation products, and many others. It seems to exist in vivo in some form not yet fully characterized. Until its pathophysiological significance, and how it is generated in vivo are determined, the nature of its true identity will be only of classical interest. In this review, its components, their biological actions and methods of preparation will be discussed.

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Acknowledgment

This work was supported by funding from National Institutes of Health, HL-069038 and HL-74239 (SP) and HL74239 (NS).

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Authors and Affiliations

  1. Division of Cardiothoracic Surgery, The Ohio State University Medical Center, Columbus, OH, USA

    Sampath Parthasarathy & Mahdi Omar Garelnabi

  2. Department of Environmental Toxicology and the Health Research Center, Southern University and A&M College, Baton Rouge, LA, USA

    Achuthan Raghavamenon

  3. Department of Pharmacology, Marshall University, Huntington, WV, USA

    Nalini Santanam

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  1. Sampath Parthasarathy
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  2. Achuthan Raghavamenon
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  3. Mahdi Omar Garelnabi
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  4. Nalini Santanam
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Editors and Affiliations

  1. A& M College, Southern University &, James L. Hunt Street, Baton Rouge, 70813, U.S.A.

    Rao M. Uppu

  2. School of Medicine, Tulane University, Tulane Ave. 1430, New Orleans, 70112, U.S.A.

    Subramanyam N. Murthy

  3. Biodynamics Inst., Louisiana State University, Baton Rouge, 70808, U.S.A.

    William A. Pryor

  4. Dorothy M. Davis Heart &, Ohio State University, West 12th Ave. 473, Columbus, 43210, U.S.A.

    Narasimham L. Parinandi

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Parthasarathy, S., Raghavamenon, A., Garelnabi, M.O., Santanam, N. (2010). Oxidized Low-Density Lipoprotein. In: Uppu, R., Murthy, S., Pryor, W., Parinandi, N. (eds) Free Radicals and Antioxidant Protocols. Methods in Molecular Biology, vol 610. Humana Press. https://doi.org/10.1007/978-1-60327-029-8_24

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